VRBGA Agar – Violet Red Bile Glucose Medium for Enterobacteriaceae Detection
Violet Red Bile Glucose Agar (VRBGA) – Gold Standard for Enteric Bacteria Isolation
Precisely isolate and identify glucose-fermenting Gram-negative bacteria with our selective VRBGA medium – the definitive choice for food safety testing, pharmaceutical QC, and environmental monitoring. Formulated with bile salts and dual chromogens for superior selectivity in detecting Enterobacteriaceae per ISO 21528 & FDA BAM standards.
Critical Applications:
✔️ Food Pathogen Screening – Salmonella/E.coli detection in raw materials
✔️ Pharmaceutical Water Testing – Indicator organism monitoring
✔️ Environmental Swab Analysis – Processing surface contamination checks
✔️ Dairy Product QC – Coliform enumeration in milk & cheese
Dual-Inhibitory Formula (per liter):
| Component | Quantity | Function |
|—————————-|———-|——————————|
| Pancreatic Digest of Gelatin| 7.0g | Peptide Nutrient Base |
| Yeast Extract | 3.0g | Vitamin Complex |
| Bile Salts | 1.5g | Gram-positive Inhibition |
| Sodium Chloride | 5.0g | Osmotic Balance |
| Glucose Monohydrate | 10.0g | Fermentation Substrate* |
| Neutral Red | 0.03g | Acid Production Indicator |
| Crystal Violet | 0.002g | Selective Inhibitor |
| High-Clarity Agar | 15.0g | Matrix Formation |
pH: 7.4 ± 0.2 (25°C) – Optimized for enteric bacteria
Glucose fermentation produces pink-red colonies with bile precipitate halos
Validated Protocol:
Dissolve 41.5g in 1L purified water
Boil 1 minute with stirring
Cool to 45-50°C (DO NOT AUTOCLAVE)
Pour Plates immediately – 4mm thickness recommended
