Sealed container of soybean-casein digest agar powder

TSA Agar Plates Medium – Soybean-Casein Digest for Sterility Testing & Bacterial Culture

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Premium Tryptic Soy Agar (TSA) – Reliable Solid Medium for Sterility Testing & Microbial Cultivation

Accurate colony counting and microorganism isolation start with our laboratory-grade Tryptic Soy Agar (TSA). This soybean-casein digest solid medium delivers uncompromised performance for sterility testing, environmental monitoring, and clinical diagnostics – formulated to meet global pharmacopoeia standards.

Key Applications:

  • ✔️ USP/EP-Compliant Sterility Testing

  • ✔️ Bacterial Colony Enumeration & Isolation

  • ✔️ Environmental Surface Monitoring (Contact Plates)

  • ✔️ Microbial Limit Testing (MLT)

  • ✔️ Long-term Culture Preservation

Optimized Formula for Robust Growth (per liter):

| Component | Quantity | Role |

|——————————-|———-|————————–|

| Pancreatic Digest of Casein | 15.0g | Primary Nitrogen Source |

| Papain Digest of Soybean Meal | 5.0g | Amino Acids/Growth Factors|

| Sodium Chloride | 5.0g | Osmotic Regulation |

| High-Purity Agar | 15.0g | Optimal Gel Strength |

pH: 7.3 ± 0.2 (25°C) – Ideal for broad-spectrum microorganism growth

Simplified Preparation Protocol:

  1. Dissolve 40.0g powder in 1L distilled/deionized water

  2. Heat & Mix: Boil with stirring until complete dissolution

  3. Sterilize:

    • 121°C for 15 minutes or

    • 115°C for 30 minutes

  4. Critical Step: Swirl gently post-sterilization to prevent agar sedimentation

  5. Pour Plates: Cool to 45-50°C before dispensing

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